Gel permeation chromatography of proteins in partly aqueous eluents
نویسندگان
چکیده
منابع مشابه
High Performance Gel Permeation Chromatography of Polymers
The determination of the molecular weight distribution by gel permeation chromatography requires the establishment of a molecular weight calibration and efficient columns in order to minimise chromatogram broadening. The separation process is described in terms of steric exclu— sion, retardation and partial exclusion mechanisms in order to show how the universal calibration method may be influe...
متن کاملArginine as an effective additive in gel permeation chromatography.
A major problem in gel permeation chromatography (GPC) or size exclusion chromatography is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as the GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody and recombinant human activin, interleukin-6, basic fibroblast growth factor, and...
متن کاملSolubility of Buffers in Aqueous–Organic Eluents for Reversed-Phase Liquid Chromatography
Address correspondence to P.W. Carr. hen preparing binary eluents containing both an organic solvent and a buffer for a buffer reversed-phase liquid chromatography (LC), one must know the solubility of the buffer in the organic cosolvent to avoid precipitation of the buffer salt. For example, one must know the highest percent organic cosolvent (that is, %B, v/v) in which a buffer salt remains s...
متن کاملDerivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis
UNLABELLED BACKGROUND The analysis of cellulose molecular weight distributions by gel permeation chromatography (GPC) is a powerful tool to obtain detailed information on enzymatic cellulose hydrolysis, supporting the development of economically viable biorefinery processes. Unfortunately, due to work and time consuming sample preparation, the measurement of cellulose molecular weight distri...
متن کاملAnalysis of pesticides in lanolin by gel permeation chromatography and gas chromatography
In this paper the efficiency of a method for the separation and identification of pesticides in lanolin is presented. Theses pesticides belong to the organophosphorous and synthetic pyrethroids families. After the gel permeation chromatography, pesticides are separated from lanolin into two fractions corresponding to the two families, which simplify subsequent analysis by gas chromatography.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 1984
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(01)96129-1